Key result
Mechanical stimulation of endothelial cells induces ~6-fold Ca2+ transients and depolarization, reducing smooth muscle Ca2+.
Why the study?
The mechanisms by which mechanical stimulation of endothelial cells affects cytosolic Ca2+ concentration and membrane potential, and its impact on co-perfused smooth muscle cells, were investigated to understand vascular relaxation responses.
Population
Cultured endothelial cells and co-perfused smooth muscle cells
Comparison
Mechanical stimulation (dimpling and poking) vs no stimulation
Design
In vitro experimental study
Authors
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Mechanical stimulation evokes Ca2+ transients and depolarization in cultured endothelial cells; hypothesis-generating for endothelial mechanotransduction, in vivo relevance remains open.
Mechanical stimulation of endothelial cells induces calcium transients and depolarization, which may serve as a mechanism for sustained vascular smooth muscle relaxation in response to increased blood flow.
Michael S. Goligorsky (1988) studied this question. Mechanical stimulation was evaluated on Cytosolic Ca2+ concentration and membrane potential. Mechanical stimulation of cultured endothelial cells induced Ca2+i transients (from 63 to 397 nM) and cell depolarization, which reduced Ca2+i in co-perfused smooth muscle cells.
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