Key result
Pre-mixing ZK 36374 with heparin impairs ADP-induced platelet inhibition but markedly enhances thrombin-induced inhibition.
Why the study?
Prostacyclin and heparin are often administered together to maximize anticoagulant effect, but their interaction on platelet function is unclear.
Does the combination of ZK 36374 and heparin alter platelet aggregation and calcium mobilization compared to either agent alone?
Population
Platelets loaded with fluorescent calcium indicator Quin 2
Comparison
ZK 36374 alone, heparin alone, and their combination
Design
Preclinical study using standard aggregometry and calcium mobilization assays
Authors
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Complex agonist-dependent interactions advise caution in combined use; leaves open translation to human therapy.
Does the combination of ZK 36374 and heparin alter platelet aggregation and calcium mobilization compared to either agent alone?
The combination of prostacyclin analogue ZK 36374 and heparin has complex, agonist-dependent interactive effects on platelet function in vitro.
Yardumian et al. (1987) studied this question. ZK 36374 and heparin vs. ZK 36374 alone and heparin alone was evaluated on Platelet aggregation and intra-platelet calcium mobilization. Pre-mixture of ZK 36374 with heparin interfered with the analogue's ability to inhibit ADP-induced platelet aggregation, but markedly enhanced its inhibition when thrombin was the agonist.
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