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October 26, 2021Journal of Cellular PhysiologyOpen Access

Role of miR‐185‐5p as modulator of periostin synthesis and smooth muscle contraction in asthma

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Key result

Modulating miR-185-5p regulates airway periostin expression and smooth muscle contraction via CDC42 and RhoA.

Why the study?

The role of miR-185-5p in regulating periostin secretion and smooth muscle contraction related to airway remodeling in asthma was not well understood.

Population

Bronchial smooth muscle cells and small airway epithelial cells from healthy subjects; sputum from healthy and asthmatic subjects

Comparison

miR-185-5p mimic and inhibitors vs control

Design

Preclinical experimental study

Authors

JRJosé M. Rodrigo‐MuñozJCJ. A. CañasBSBeatriz Sastre

Discussion

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Member takes

Overview

No immediate change to asthma care; leaves open miR-185-5p–periostin targeting for further mechanistic and clinical validation.

Structured PICO

P
Population
Bronchial smooth muscle cells (BSMCs) and small airway epithelial cells (SAECs) from healthy subjects, and sputum from healthy and asthmatic subjects
I
Intervention
miR-185-5p mimic and inhibitors
O
Outcome
Periostin (POSTN) gene expression and protein levels, CDC42 and RHOA levels, BSMC contractility, and intracellular calciumsurrogate

miR-185-5p modulates periostin secretion and smooth muscle contraction in airway structural cells, highlighting its potential as a therapeutic target in asthma.

Cite This Study

Rodrigo‐Muñoz et al. (2021) studied Asthma. miR-185-5p modulation was evaluated on Periostin secretion and smooth muscle contraction. Modulation of miR-185-5p regulated periostin expression in airway structural cells and smooth muscle contraction via CDC42 and RhoA, with diminished miR-185-5p seen in asthmatic sputum.

synapsesocial.com/papers/6aacd9a50183981edfbf81bfhttps://doi.org/10.1002/jcp.30620
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Roles of Periostin in Respiratory Disorders2016 · 184 citations
  2. 2Mutation of the mouse klotho gene leads to a syndrome resembling ageing.1997 · 66,546 citations
  3. 3Recent advances in understanding eosinophil biology2017 · 27 citations
  4. 4Interleukin-13 Augments Bronchial Smooth Muscle Contractility with an Up-Regulation of RhoA Protein2008 · 170 citations
  5. 5miRSystem: An Integrated System for Characterizing Enriched Functions and Pathways of MicroRNA Targets2012 · 375 citations