Key result
Decreasing intracellular pH to 5.8 inhibits Kir4.1 open-state probability by ~70% while increasing conductance.
Why the study?
The biophysical properties of Kir4.1 channels underlying the inhibition of whole-cell currents by decreased intracellular pH were not fully understood.
Population
Kir4.1 channels studied in inside-out patches
Comparison
Various intracellular pH levels vs baseline pH 7.4
Design
Preclinical electrophysiological study using giant inside-out patches
Authors
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Acidosis effects on Kir4.1 may alter cardiac electrophysiology; extends channel biophysics but leaves clinical relevance open.
Intracellular protons exert opposite effects on the open-state probability and single channel conductance of Kir4.1 channels, which explains the changes in macroscopic currents observed at low pH.
Yang et al. (1999) studied this question. Intracellular pH reduction vs. Baseline pH 7.4 was evaluated on Channel open-state probability (Popen) and single channel conductance. Decreasing intracellular pH to 5.8 inhibited the open-state probability of Kir4.1 channels by 70% while increasing single channel conductance by 35%.
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