July 1, 2000The Journal of General PhysiologyOpen Access

Biophysical and Molecular Mechanisms Underlying the Modulation of Heteromeric Kir4.1–Kir5.1 Channels by Co2 and Ph

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Population

Inside-out patches expressing heteromeric Kir4.1-Kir5.1 channels and homomeric Kir4.1 channels

Comparison

Exposure to varying pH levels, CO2… vs Baseline pH, absence of PIP2, and wild-type…

Design

Preclinical

Authors

ZYZhenjiang YangShenzhen Pingle Orthopedic Hospital HXHaoxing XuUniversity of Michigan NCNingren CuiPurdue University West Lafayette

Discussion

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Implication

May affect excitability in pH-altered states; leaves open clinical translation of Kir4.1-Kir5.1 modulation.

Structured PICO

Population

Inside-out patches expressing heteromeric Kir4.1-Kir5.1 channels and homomeric Kir4.1 channels

Intervention

Exposure to varying pH levels (6.5 to 8.5), CO2, phosphatidylinositol-4,5-bisphosphate (PIP2), and site-directed mutagenesis (K67M, K67Q in Kir4.1; Arg178 in Kir5.1)

Comparator

Baseline pH (7.4), absence of PIP2, and wild-type channels

Outcome

Channel open-state probability (P(open)), single channel conductance, and pH/CO2 sensitivity (pKa)surrogate

Heteromeric Kir4.1-Kir5.1 channels are modulated by both acidic and alkaline pH, with PIP2 altering pH sensitivity via specific molecular residues.

Cite This Study

Yang et al. (2000) studied this question.

synapsesocial.com/papers/6a8a5843336687a3262e5213 https://doi.org/10.1085/jgp.116.1.33

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Biophysical and Molecular Mechanisms Underlying the Modulation of Heteromeric Kir4.1–Kir5.1 Channels by Co2 and Ph

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Biophysical and Molecular Mechanisms Underlying the Modulation of Heteromeric Kir4.1–Kir5.1 Channels by Co2 and Ph

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider