What does this research mean for the field?

A rapid LC-MS/MS assay with accelerated enzymatic digestion achieves high analytical linearity and reproducibility for HbA1c quantification in whole blood, correlating strongly (r = 0.998) with automated clinical analyzers. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

October 26, 2024Analytical and Bioanalytical ChemistryOpen Access

Early developments toward HbA1c determination in whole blood by high-speed sample preparation and LC–MS/MS analysis

Q: What is the clinical evidence from this study?

Study design: Other. Population: HbA1c determination. Intervention: LC-MS/MS method with fast hemolysis and digestion vs. Roche Cobas c 513 clinical analyzer. Primary outcome: Correlation with clinical analyzer (correlation coefficient 0.998).

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Key result

Rapid LC-MS/MS method correlates tightly with standard clinical analyzer for measuring HbA1c.

correlation coefficient 0.998

Population

Whole blood samples

Comparison

Novel high-speed sample preparation and LC-MS/MS analysis vs Roche Cobas c 513 clinical analyzer

Design

Analytical method development and validation study

Authors

IMIndranil Mitra ALAndreas LeinenbachUniversity of Augsburg AGAndrea GeistangerRoche Pharma AG (Germany)

Discussion

Member takes

Implication

A novel LC-MS/MS method for HbA1c determination offers rapid sample preparation and measurement while maintaining analytical performance comparable to traditional clinical analyzers.

Structured PICO

Population

Whole blood samples

Intervention

Fast hemolysis, digestion with 0.6 mg/mL endoproteinase Glu C, and LC-MS/MS analysis with a run time of 36 s

Comparator

Roche Cobas® c 513 clinical analyzer

Outcome

HbA1c determination (linearity, reproducibility, and correlation with clinical analyzer)surrogate

Main Result

Effect estimate: correlation coefficient 0.998

A novel LC-MS/MS method for HbA1c determination offers rapid sample preparation and measurement while maintaining analytical performance comparable to traditional clinical analyzers.

Cite This Study

Mitra et al. (2024) studied HbA1c determination. LC-MS/MS method with fast hemolysis and digestion vs. Roche Cobas c 513 clinical analyzer was evaluated on Correlation with clinical analyzer (correlation coefficient 0.998). A rapid LC-MS/MS method for HbA1c determination demonstrated high linearity and reproducibility, correlating strongly (r=0.998) with the Roche Cobas c 513 clinical analyzer.

synapsesocial.com/papers/6aace67ce1d6435567477974 https://doi.org/10.1007/s00216-024-05601-5

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Implication

A novel LC-MS/MS method for HbA1c determination offers rapid sample preparation and measurement while maintaining analytical performance comparable to traditional clinical analyzers.

Early developments toward HbA1c determination in whole blood by high-speed sample preparation and LC–MS/MS analysis

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Early developments toward HbA1c determination in whole blood by high-speed sample preparation and LC–MS/MS analysis

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider