Samples of domestically distributed soybeans from Brazil produced in 2021 and 2022 were analyzed using 17 event-specific and 2 screening detection methods for genetically modified (GM) soybeans. We found that the detected varieties of GM soybeans from Brazil were significantly different from those observed in our previous study on GM soybeans imported from the United States and Canada within the same years [Soga et al., GM Crops & Food, 16, 116-125 (2025)]. After analyzing five different lots for each year, RRS, MON89788, and MON87701 were detected in all lots, whereas MON87708 and MON87751 were detected in four lots. RRS, MON89788, MON87701, MON87708 and MON87751 are a glyphosate tolerant, a glyphosate tolerant, an insect resistant, a glyphosate and dicamba tolerant, and an insect resistant GM soybean, respectively. Single kernel-based analyses revealed that stacked varieties, including MON89788 and MON87701, comprised a large proportion of GM soybeans from Brazil.
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