Key result
Capillary electrophoresis with laser-induced fluorescence detects anthracyclines at levels below ~1 ng/mL.
Why the study?
The simultaneous determination of structurally similar anthracycline antibiotics doxorubicin, daunorubicin, and idarubicin required development of an efficient and stable analytical method.
The developed capillary electrophoresis method allows for the efficient and sensitive simultaneous determination of doxorubicin, daunorubicin, and idarubicin in serum samples.
The separation and simultaneous determination of doxorubicin, daunorubicin and idarubicin was investigated using capillary electrophoresis with laser-induced fluorescence detection. Because the three anthracycline antibiotics were similar in structure and mass, careful manipulation of the electroosmotic flow and electrophoretic mobilities was required. A buffer consisting of 100 mM borate, adjusted to pH 9.5, containing 30% acetonitrile was found to provide a very efficient and stable electrophoretic system for the analysis of the three anthracyclines. The method was applied to the determination of three anthracyclines in serum samples. Responses were linear in the range of 10-500 ng.mL-1 and the detection limits were lower than 0.9 ng.mL-1.
No takes yet. Share an insight, caveat, or question.
Pérez‐Ruiz et al. (2001) studied this question. Capillary electrophoresis with laser-induced fluorescence detection was evaluated on Linearity and detection limits for anthracyclines in serum. Capillary electrophoresis with laser-induced fluorescence detection provided linear responses for anthracyclines in the range of 10-500 ng/mL with detection limits below 0.9 ng/mL.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: