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Adrenal corticosteroid concentrations were determined by a competitve proteinbinding technique in blood samples taken at various times of the day via indwelling jugular cannulae from six cows in each of three groups, pregnant nonlactating, nonpregnant lactating, and pregnant lactating.The results showed a pattern of plasma corticosteroid concentration suggestive of a daily rhythm with higher concentrations between 2:30 and 6:30 A~ and lower concentrations at 8:30 PM in the three groups.Pregnancy and lactation status had no signi~cant effect on the daily pattern, but the daily cortieosteroid mean was higher in the pregnant, lactating group (.64 ± .05/~g/100 ml; mean ___ SE) than in the pregnant, nonlactating group (.50 ___ .03/xg/100ml).
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MacAdam et al. (1972) studied this question.
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