Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco

Fig 1. A. Liver with very severe hyperemia, severe necrosis of individual/groups of hepatocytes and the presence of basophilic intra-nuclear inclusion bodies within hepatocytes. H&E, X400. B. Liver of SPF- chicken embryo inoculated with field liver homogenates showing multifocal necrosis of groups of hepatocytes and the presence of basophilic inclusions bodies (intra-nuclear) within hepatocytes. H&E, X40. C. Liver of SPF chicken embryo (higher magnification of Fig B)–foci of hepatocytes necrosis. Cellular debris and basophilic intra-nuclear inclusion bodies. H&E, X160.

Fig 2. Cytopathic effect of FAdV in CEF cells. A) Eighty percent confluent CEF cells. B) Typical cytopathic effects shown as cell rounding, refractility and detachment.

Fig 3. Isolation of FAdV using embryonated chicken eggs. An infected embryo shows curling and dwarfing 4 days after inoculation in comparison to the control embryo.

Fig 4. Inoculated embryo with liver enlarged and showed a diffuse greenish discolouration.

Fig 5. Hexon gene DNAs of fowl aviadenovirus detected from samples, Lane 1: Homogenized liver tissues (Positive control). Lane 2: Inoculated embryos. Lane 3: Extraction negative control. Lane 4: Amplification negative control. Lane 5: Supernatant of second passage of infected cell culture. Lane 6: Supernatant from third passage of infected cell culture. Lane M: Molecular weight 100 pb.

Fig 6. Phylogenetic tree based on nucleotide sequence of hexon gene of FAdV field isolates and sequences from gene bank.