Key result
Slow Ca2+ channel sensitivity to isoproterenol in rat cardiomyocytes jumps sharply around fetal day 19.
Why the study?
Developmental changes in beta-adrenergic and muscarinic modulations of slow Ca2+ channels in rat ventricular cardiomyocytes were not fully characterized.
Population
Single ventricular cardiomyocytes freshly isolated from fetal and neonatal rats
Comparison
Isoproterenol (1 microM), forskolin (10 microM), and dose-dependent carbachol effects on IBa(L)
Design
Preclinical electrophysiological study using whole-cell voltage clamp
Authors
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Highlights critical window of beta-adrenergic maturation in rat heart; hypothesis-generating for human fetal cardiac development.
Beta-adrenergic modulation of calcium currents in rat ventricular cardiomyocytes undergoes a significant developmental increase during the late fetal period (days 18-20).
Masuda et al. (1996) studied Fetal and neonatal rat ventricular cardiomyocytes. Isoproterenol, forskolin, and carbachol vs. Basal state across different developmental days was evaluated on Modulation of slow Ca2+ channels (IBa(L)). Beta-adrenergic modulation of slow Ca2+ channels in rat ventricular cardiomyocytes demonstrated a large jump in sensitivity to isoproterenol around fetal day 19.
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