Synthesis and Biological Activities Evaluation of Novel Spiroheterocycles Containing 1,3,4-Oxadiazoline Moiety

A novel series of spiro 3-acetyl-1,3,4-oxadiazolines were synthesized via oxidative cyclization reaction of different 2-furoyl and 2-thenoyl hydrazones with acetic anhydride. The structures of obtained compounds were confirmed by IR, MS, 1H NMR, 13C NMR and Elemental analysis methods and are in full agreement with their molecular structure. The newly synthesized spiro 1,3,4-oxadiazolines were screened for in vitro for their biological activity against a variety of bacterial strains (Euterococci, Escherichia coli, Staphylococcus aureus, Klebsiella spp, Proteus spp), and fungi (Aspergillus niger, Candida albicans), employing the nutrient agar disc diffusion method. The obtained results showed that these compounds have good inhibition against the tested pathogens.