Key result
Automated protocol quantifies ventricular collagen and lipofuscin from label-free microscopy to avoid observer discrepancies.
Why the study?
Routine procedures for collagen and lipofuscin evaluation in human ventricular tissue are often qualitative, observer dependent, and ignore spatial distribution.
This protocol enables standardized, observer-independent quantification of myocardial remodeling features in human ventricular tissue.
Answers are grounded in this paper and cite their sources.
May standardize fibrosis quantification in ventricular tissue; leaves open validation before research or clinical adoption.
The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species. For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022).1
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García‐Mendívil et al. (2023) studied Myocardial remodeling. Automatic quantification protocol from label-free microscopy vs. Routine qualitative procedures was evaluated on Quantification and spatial characterization of collagen and lipofuscin. A protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues was developed to avoid observer discrepancies.
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