Key result
Indirect ELISA and IFA assays detect murine norovirus antibodies in ~58% of conventional mouse samples.
Why the study?
Murine norovirus infection prevalence in laboratory mice requires evaluation through development of reliable antibody detection assays.
An indirect ELISA and IFA assay were successfully developed to detect murine norovirus S7 antibodies, revealing a high prevalence of the infection in conventional mouse colonies in Japan.
High MNV prevalence supports routine screening in conventional colonies; leaves open effects on cardiovascular mouse model validity.
To evaluate murine norovirus (MNV) infection in laboratory mice, we attempted to develop an enzyme-linked immunosorbent assay (ELISA) system and an indirect immunofluorescent antibody (IFA) assay for detecting the anti-MNV-S7 antibody in mice. MNV-S7, which was isolated in Japan, was used in both assays. The antigen for ELISA was prepared by ultracentrifugation of culture supernatants of RAW 264 cells infected with MNV-S7. Positive sera were obtained from 6-week-old, female C57BL/6JJcl mice inoculated orally with MNV-S7. IFA against infected RAW 264 cells was able to discriminate positive sera from negative sera. Indirect ELISA was performed using 96-well ELISA plates coated with formalin-treated MNV-S7 antigen. In this ELISA system, mouse sera obtained 2 weeks after infection or later showed significantly high OD values and were judged positive. An equal level of anti-MNV-S7 antibody response was observed in BALB/cAJcl, C57BL/6JJcl, DBA/2JJcl, and Jcl:ICR mice; whereas, C3H/HeJJcl mice demonstrated slightly lower antibody production 4 weeks after infection. We also used this ELISA system to evaluate 77 murine serum samples obtained from 15 conventional mouse rooms in research facilities in Japan and found that approximately half of the serum samples contained antibody to MNV-S7. We found that some serum samples were negative for antibodies to mouse hepatitis virus and Mycoplasma pulmonis but positive for antibody to MNV-S7. The results suggest that the MNV infection is more prevalent than other infections such as mouse hepatitis virus and Mycoplasma pulmonis in conventional mouse colonies in Japan, as is the case in other areas of the world.
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KITAGAWA et al. (2010) studied Murine norovirus (MNV) infection (n=77). Indirect ELISA and IFA vs. Naive mouse sera was evaluated on Prevalence of anti-MNV-S7 antibodies in conventional mouse colonies. An indirect ELISA and IFA system successfully detected anti-MNV-S7 antibodies, revealing that 58.4% of 77 serum samples from conventional mouse colonies in Japan were positive for the murine norovirus.
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