Key result
Lactated Ringer's reperfusion reduces cardiac apoptosis and increases survival markers in shocked diabetic rats.
Why the study?
Diabetes causes organ damage and the effects of reperfusion using lactate Ringer’s solution in diabetic hemorrhagic shock were investigated.
Does reperfusion using lactate Ringer's solution reduce cardiac apoptosis in diabetic rats with hemorrhagic shock?
Does reperfusion using lactate Ringer's solution reduce cardiac apoptosis in diabetic rats with hemorrhagic shock?
Reperfusion with lactate Ringer's solution may mitigate cardiac damage and apoptosis following hemorrhagic shock in diabetic models by blocking the IGF II R pathway.
Answers are grounded in this paper and cite their sources.
May not yet alter clinical reperfusion strategies in diabetic shock; hypothesis-generating for lactate Ringer's in animal models.
Diabetes is a metabolic disorder that damages many organs. We investigated the effects of reperfusion using lactate Ringer’s solution (LR) in a diabetic animal model. Eight-week-old rats were divided into groups: control, hemorrhagic shock induced (HS), diabetes mellitus (DM), DM plus HS (DM + HS) and DM rats that received LR after HS (DM + HS + LR). HS was induced by withdrawing blood from the femoral artery and arterial pressure was maintained at 40 mm Hg for 1 h. Animals were perfused with either withdrawn blood or LR. Rats were sacrificed and hearts were collected from all groups. Histopathological studies were performed using left ventricles and western blotting analysis was performed using protein extracted from the left ventricle. Using the TUNEL assay, we found more apoptotic cells in the DM + HS group compared to the control group, whereas in animals resuscitated with LR, the number of apoptotic cells was reduced. Western blotting showed a significant reduction in apoptotic markers, cyt c, cas 9 and cas 3, and increased survival markers, pPI3K and pAKT, in the DM + HS + LR group. Reperfusion with LR may have therapeutic effects on trauma induced HS by blocking the IGF II R facilitated apoptosis pathway in diabetic rats.
No takes yet. Share an insight, caveat, or question.
Lin et al. (2020) studied Diabetes mellitus and hemorrhagic shock. Lactate Ringer's solution vs. Withdrawn blood / control groups was evaluated on Apoptotic cells and markers (cyt c, cas 9, cas 3) and survival markers (pPI3K, pAKT). Reperfusion with lactate Ringer's solution significantly reduced apoptotic markers and increased survival markers in diabetic rats subjected to hemorrhagic shock.
Synapse has enriched 10 related papers. Consider them for comparative context: