Key result
New purification protocol yields pure recombinant RyR protein suitable for single-channel functional studies.
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Describes a purification protocol for recombinant Ryanodine Receptors expressed in HEK293 cells for use in functional studies.
, 2020 ). This purification protocol is also suitable for functional studies, such as single-channel analysis, that require pure RyR protein.
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Hu et al. (2021) studied this question. A purification protocol for recombinant wild-type and mutant ryanodine receptors expressed in HEK293 cells yields pure RyR protein suitable for functional studies like single-channel analysis.
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