January 31, 1975Science
Type C RNA Tumor Virus Isolated from Cultured Human Acute Myelogenous Leukemia Cells
Key result
Culturing leukocytes from a patient with acute myelogenous leukemia in conditioned media enabled the continuous production and isolation of budding type C RNA tumor viruses.
Population
Cultured human acute myelogenous leukemia cells
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May indicate retroviral involvement in AML; hypothesis-generating and should not yet change practice.
Structured PICO
P
Population
Leukocytes from a single patient with acute myelogenous leukemia cultured in liquid suspension.
E
Exposure
Culturing in liquid suspension culture using a source of conditioned media
O
Outcome
Continuous production of budding type C viruses with characteristic reverse transcriptase
The study reports the successful isolation and continuous production of a type C RNA tumor virus from cultured human acute myelogenous leukemia cells.
Cite This Study
Gallagher et al. (1975) studied Acute myelogenous leukemia (n=1). Culturing in conditioned media was evaluated on Continuous production of budding type C viruses. Culturing leukocytes from a patient with acute myelogenous leukemia in conditioned media enabled the continuous production and isolation of budding type C RNA tumor viruses.