Key result
Atypical porcine pestivirus was detected in 90.4% of piglets with congenital tremors across 12 farms in Southern China, with 62.5% of sequenced strains belonging to a novel subgenotype provisionally named genotype 3.
Observational (n=83)
Yes
A novel APPV subgenotype (genotype 3) is widely distributed in piglets with severe congenital tremors in Southern China.
Surveillance for APPV warranted in Southern China swine farms; leaves open full genetic diversity, evolution, and control needs for congenital tremor.
Atypical porcine pestivirus (APPV) is an RNA virus newly discovered from swine in Asia, Europe, and the Americas. This novel virus has been confirmed as the cause of congenital tremor (CT) in piglets, which causes extensive economic losses to the swine industry. To investigate the genetic diversity and evolutionary relationship of APPV in China, 83 piglet samples with severe CT clinical signs were obtained from 12 commercial swine farms in 3 provinces of Southern China. RT-PCR revealed that the positive rates of APPV were as high as 100% (12/12) for the swine farms and 90.4% (75/83) for the samples. Subsequently, 21 positive samples and 3 positive samples were selected for partial E2 gene and complete polyprotein gene sequencing, respectively. Phylogenetic analysis showed that 62.5% of the sequences belonged to a novel APPV clade provisionally named genotype 3, which showed 81.0-82.1% sequence identity to genotypes 1 and 2. Amino acid sequence alignment showed that E2 protein of genotype 3 has three specific mutation sites, namely I19V, Y82F, and N107G. The results of the present study demonstrate that a novel APPV subgenotype, which is widely distributed in severe CT clinical samples in Southern China, was genetically diverse. We advocate for the inclusion of genotype 3 during revision of the APPV typing method.
No takes yet. Share an insight, caveat, or question.
Xie et al. (2019) conducted an observational in Congenital tremor (n=83). Atypical porcine pestivirus (APPV) was evaluated on Detection rate of APPV by RT-PCR. Atypical porcine pestivirus was detected in 90.4% of piglets with congenital tremors across 12 farms in Southern China, with 62.5% of sequenced strains belonging to a novel subgenotype provisionally named genotype 3.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: