What question did this study set out to answer?

Certify the catalytic activity concentrations of four reference enzyme preparations (GGT, LDH 1, ALT, and CK) at 37°C using IFCC primary reference methods.

January 24, 2002Clinical Chemistry and Laboratory Medicine (CCLM)

IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C. Part 7. Certification of Four Reference Materials for the Determination of Enzymatic Activity of γ-Glutamyltransferase, Lactate Dehydrogenase, Alanine Aminotransferase and Creatine Kinase according to IFCC Reference Procedures at 37°C

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Authors

LSLothar SiekmannUniversity Hospital Bonn RBR BonoraUniversity of Bonn CBC A BurtisOak Ridge National Laboratory

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Implication

Collaborative standardization study establishes certified catalytic activity concentrations for four enzyme reference materials at 37°C, highlighting improved traceability for routine diagnostic...

Key Points

Certify the catalytic activity concentrations of four reference enzyme preparations (GGT, LDH 1, ALT, and CK) at 37°C using IFCC primary reference methods.
Assessed material homogeneity and stability using historical and newly generated testing data.
Conducted an international collaborative study under strict quality control to assign values and calculate measurement uncertainty in accordance with GUM guidelines.
Certified catalytic activity concentrations at 37°C for γ-glutamyltransferase (IRMM/IFCC 452) at 1.90 ± 0.04 µkat/l (114.1 ± 2.4 U/l) and lactate dehydrogenase 1 (IRMM/IFCC 453) at 8.37 ± 0.12 µkat/l (502 ± 7 U/l).
Certified values at 37°C for alanine aminotransferase (IRMM/IFCC 454) at 3.09 ± 0.07 µkat/l (186 ± 4 U/l) and creatine kinase (IRMM/IFCC 455) at 1.68 ± 0.07 µkat/l (101 ± 4 U/l).

Cite This Study

Siekmann et al. (2002) studied this question.

synapsesocial.com/papers/6ab243c136566efabda038fb https://doi.org/10.1515/cclm.2002.127

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Implication

Collaborative standardization study establishes certified catalytic activity concentrations for four enzyme reference materials at 37°C, highlighting improved traceability for routine diagnostic...

Discussion

Member takes

Implication

Key Points

Cite This Study

Discussion

Member takes

Implication

Key Points

Cite This Study

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