Key result
Oral Val-Pro-Pro and Ile-Pro-Pro accumulate in vascular endothelial cells, suggesting in vivo ACE inhibition.
Why the study?
The in vivo accumulation and localization of antihypertensive peptides Val-Pro-Pro and Ile-Pro-Pro in vascular endothelial cells were not established.
The accumulation of orally administered Val-Pro-Pro and Ile-Pro-Pro in vascular endothelial cells provides a mechanistic basis for their in vivo ACE inhibitory activity.
Endothelial localization in rats supports ACE-inhibitory mechanism; leaves open human translation and clinical relevance.
The antihypertensive peptides, Val-Pro-Pro and Ile-Pro-Pro, were successfully detected in the aorta of spontaneously hypertensive rats after orally administering these peptides by a guanidine-thiocyanate treatment to prevent proteolysis. Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme.
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Kawaguchi et al. (2012) studied Hypertension (in rats). Val-Pro-Pro and Ile-Pro-Pro was evaluated on Detection and localization of peptides in the aorta. Oral administration of Val-Pro-Pro and Ile-Pro-Pro in spontaneously hypertensive rats resulted in their accumulation in vascular endothelial cells, suggesting in vivo ACE inhibitory activity.
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