Key result
Platelet adhesion on glass spontaneously drives procoagulant and aggregating subpopulations across all platelets.
Why the study?
The regulation of formation of platelet subpopulations upon activation remains unclear despite evidence of distinct procoagulant and aggregating platelet subpopulations.
This study demonstrates that all platelets have the capacity to express multiple activation markers, suggesting that observed subpopulations reflect the dynamics of the activation process rather than distinct lineages.
Answers are grounded in this paper and cite their sources.
Does not alter clinical platelet testing; leaves open whether activation dynamics drive heterogeneity in human thrombosis.
Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been reported in the literature in platelets treated with the GPVI agonists. How the formation of platelet subpopulations upon activation is regulated remains unclear. Here, it is shown that procoagulant and aggregating platelet subpopulations arise spontaneously upon adhesion of purified platelets on clean glass surfaces. Calcium ionophore treatment of the adhering platelets resulted in one platelet population expressing both the procoagulant and the adherent population markers phosphatidylserine and the activated form of GPIIb/IIIa, while all of the platelets expressed CD62P independently of the ionophore treatment. Therefore, all platelets have the capacity to express all three activation markers. It is concluded that platelet subpopulations observed in various studies reflect the dynamics of the platelet activation process.
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Donati et al. (2016) studied Platelet activation. Calcium ionophore treatment was evaluated on Expression of activation markers (phosphatidylserine, activated GPIIb/IIIa, CD62P). Procoagulant and aggregating platelet subpopulations arise spontaneously upon adhesion on clean glass surfaces, demonstrating that all platelets have the capacity to express all activation markers.
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