What question did this study set out to answer?

To determine whether C-reactive protein binds human polymorphonuclear leukocytes via Fc receptors and stimulates cellular responses analogous to IgG immune complexes.

March 1, 1986The Journal of Immunology

Binding of C-reactive protein to human polymorphonuclear leukocytes: evidence for association of binding sites with Fc receptors.

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Authors

HMH.‐Arno J. MüllerUniversity of Kassel JFJan FehrPrevention Institute

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Implication

In vitro study reveals C-reactive protein binds polymorphonuclear leukocytes via Fc receptors in plasma, indicating a targeted role in localizing inflammation without triggering cytotoxic damage.

Key Points

To determine whether C-reactive protein binds human polymorphonuclear leukocytes via Fc receptors and stimulates cellular responses analogous to IgG immune complexes.
Measured binding kinetics and saturability using purified 125I-labeled CRP incubated with human polymorphonuclear leukocytes (PMN) in heat-inactivated plasma.
Performed competitive binding assays using antigen-complexed and aggregated IgG to evaluate Fc receptor involvement.
Assessed PMN surface adherence, aggregation, secondary granule release, and activation of the hexose monophosphate pathway.
125I-labeled CRP demonstrated specific and saturable binding to PMN exclusively in heat-inactivated plasma, with a KD of 10.5 ± 5.7 × 10⁻⁸ M and an estimated 1 to 3 × 10⁶ binding sites per cell.
Binding of CRP was competitively inhibited by antigen-complexed or aggregated IgG, indicating that CRP binding sites are associated with PMN Fc receptors.
CRP binding induced cell adherence to culture surfaces in a plasma-dependent manner, but did not trigger PMN aggregation, secondary granule release, or hexose monophosphate pathway activation.

Cite This Study

Müller et al. (1986) studied this question.

synapsesocial.com/papers/6ab4be7e929cbb03e89ef4fc https://doi.org/10.4049/jimmunol.136.6.2202

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Implication

In vitro study reveals C-reactive protein binds polymorphonuclear leukocytes via Fc receptors in plasma, indicating a targeted role in localizing inflammation without triggering cytotoxic damage.

Binding of C-reactive protein to human polymorphonuclear leukocytes: evidence for association of binding sites with Fc receptors.

Discussion

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Implication

Key Points

Cite This Study

Binding of C-reactive protein to human polymorphonuclear leukocytes: evidence for association of binding sites with Fc receptors.

Discussion

Member takes

Implication

Key Points

Cite This Study

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