Spoilage can be evaluated by separating and determining biogenic amines by various techniques, notably high-performance liquid chromatography. Previous studies have not taken into account how the muscle tissue matrix affects the assay. We demonstrate a matrix effect in plaice and whiting and show that it changes during spoilage. This effect should be taken into account when plotting regression lines relating the quantity of amine to the biogenic amine/internal standard ratio.
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Duflos et al. (1999) studied this question.