Key result
An attenuated mutant strain of Japanese encephalitis virus produced no viremia or clinical symptoms in pigs while evoking persistent hemagglutination-inhibiting and neutralizing antibodies.
Why the study?
Does an attenuated mutant strain of Japanese encephalitis virus produce immunity without causing clinical symptoms in swine?
Does an attenuated mutant strain of Japanese encephalitis virus produce immunity without causing clinical symptoms in swine?
An attenuated mutant strain of Japanese encephalitis virus safely induces a persistent immune response in swine without causing viremia or clinical disease.
Colostrum-deprived pigs enable sensitive JE virulence assays in swine; leaves open translation to field infections or vaccine studies.
Summary Colostrum-deprived pigs were found to be highly susceptable to Japanese encephalitis (JE) virus even by subcutaneous inoculation. Such animals represent the most suitable host for assaying the virulence of JE virus in swine. When a small dose (10 LD50) of a virulent strain was injected subcutaneously in colostrum-deprived pigs, they showed significant viremia and clinical signs, including nervous symptoms. On the other hand, an attenuated mutant (m) strain of JE virus produced no detectable viremia and no clinical symptoms even when more than 107 TCD50 of the virus was inoculated. The m virus was able to evoke both hemagglutination-inhibiting (HI) and neutralizing antibodies in colostrum-deprived pigs after a single subcutaneous injection. The same antibody responses were also observed after one injection of m virus in young adult pigs with HI antibody titers less than 1:10, and the antibody levels persisted for at least 80 days.
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Kodama et al. (1968) studied Japanese encephalitis. Attenuated mutant (m) strain of JE virus vs. Virulent strain of JE virus was evaluated on Viremia, clinical symptoms, and antibody responses (HI and neutralizing antibodies). An attenuated mutant strain of Japanese encephalitis virus produced no viremia or clinical symptoms in pigs while evoking persistent hemagglutination-inhibiting and neutralizing antibodies.
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