Apoptosis and Mobilization of Lymphocytes to Cardiac Tissue Is Associated with Myocardial Infarction in a Reperfused Porcine Model and Infarct Size in Post‐PCI Patients

Figures

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  Figure 1. Flowchart of the study group. This flowchart illustrates the STEMI patients and controls and the different blood samples that were obtained. In total, 116 STEMI patients were selected after exclusion criteria: 18 STEMI patients were selected for serial blood extractions at different time points and 98 STEMI patients were selected for 24 h post-PCI blood extraction and CMR during 1st week and after 6 months. STEMI: ST-segment elevation myocardial infarction; CMR: cardiac magnetic resonance.

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  Figure 2. Lymphocyte and T cell dynamics in swine with induced infarction. Dynamics of lymphocyte and T cell subsets in blood from swine with induced infarction at different time points: preinfarction (0 h), postinfarction and prereperfusion (90 min), and postreperfusion (2 h and 48 h). (a) Lymphocyte cell count. (b) Lymphocyte apoptosis. Number of Annexin V-positive cells selected in the lymphocyte gate. (c) T cells (CD3). (d) B cells (CD1). (e) CD8 cells. (f) CD4 cells. (g) Treg cells (CD4+CD25+FOXP3+). Data are expressed as mean ± SD. versus preinfarction (0 h). SD: standard deviation; Treg: T regulatory cells.

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  Figure 3. T cell infiltration and lymphocyte apoptosis in swine myocardial samples. (a) Microscopic captions of porcine myocardium; top: T cell (CD3) infiltration; bottom: B cell (CD79a) in infarcted, adjacent, and remote areas. (b) T cell subset gene expression: FOXP3, GATA 3, and TBETX in infarcted, adjacent, and remote areas. Data are expressed as mean ± SD. ∗ p < 0.05 versus remote. SD: standard deviation.

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  Figure 4. Lymphocyte and T cell dynamics STEMI patients. Total lymphocyte, lymphocyte apoptosis (Annexin V+ cells), and T cell subset count (cells/μl) from blood of STEMI patients at different time points: after MI and before reperfusion (pre-PCI) and after MI and after reperfusion (24 h, 96 h, and 30 d post-PCI). A significant drop of lymphocytes, T cell (CD3+), CD4, CD8, and Th1 (CD4+CXCR3+) 24 h post-PCI was observed in STEMI patients. While B cells (CD19+) and Th2 (CD4+CCR4+) did not change. Treg (CD4+CD25+FOXP3+) increased after one month. Values are expressed as mean ± SD. p < 0.05 or p < 0.01 versus pre-PCI. PCI: percutaneous coronary intervention; SD: standard deviation; STEMI: ST-segment myocardial infarction; Treg: T regulatory cells.

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  Figure 5. CD4 T cell subsets dynamics in STEMI patients. mRNA gene expression analysis of the dynamics of different CD4 subsets in STEMI patients. Tbetx gene expression indicates Th1 cells, Gata3 gene expression indicates Th2 cells, and FOXP3 gene expression indicates Treg cells. A significant decrease of proinflammatory Th1 cells was observed 24 h post-PCI while Treg cells increased within the next month. Values are expressed as mean ± SD. p < 0.05 or p < 0.01 versus pre-PCI. PCI: percutaneous coronary intervention; SD: standard deviation; STEMI: ST-segment myocardial infarction.

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  Figure 6. Association of CMR-derived infarct size with T cell count in STEMI patients 24 h post-PCI. (a) Example of CMR images from a patient with an anterior STEMI. (b) Association of 1-week CMR-derived infarct size with T cell count (cells/μl) in blood of STEMI patients at 24 h post-PCI. (c) Association of 6-month CMR-derived infarct size with T cell count (cells/μl) in blood of STEMI patients at 24 h post-PCI. (d) Association of 1-week CMR-derived infarct size with T cell subsets (cells/μl) in blood of STEMI patients at 24 h post-PCI. (e) Association of 6-month CMR-derived infarct size with T cell subsets (cells/μl) in blood of STEMI patients 24 h post-PCI. Data are expressed as mean ± SD. ∗ p < 0.05. PCI: percutaneous coronary intervention; SD: standard deviation; STEMI: ST-segment myocardial infarction.