Arabidopsis Ascorbate Oxidase Mutant Characterization to Determine its Physiological Role in Plant Development

The T-DNA insertional mutant assemblies of genes of Arabidopsis thaliana are making a significant contribution toresearch in plant biology since past two decades. A broad-spectrum of problem in plant biology intended at in-vivo genecharacterization for various functions were being studied by these mutant alleles. The study was undertaken to appraisethe regulatory role of ascorbate oxidase gene in plant development to see its role in reactive oxygen species. To evaluatethe functional role of ascorbate oxidase a mutant lacking AO was compared to the wild-type. For this purpose, a salk-linehomozygous for an insertion in the AO coding sequence was used and verified for the presence of the insertionalmutagenesis. Much effort needs to complete this picture, and ultimately fulfill the present-day gap in our judgement ofsuch potential instigator of plant responses.