Key result
Dantrolene preserves calcium transients to ~89% of control after deep hypothermic rewarming.
Why the study?
The study investigated how different degrees of hypothermia affect cardiac muscle function and the role of the RyR(2) channel in hypothermia-induced changes in myoplasmic calcium.
Does deep versus moderate hypothermia affect excitation-contraction coupling and calcium transients in rat ventricular myocytes, and does dantrolene mitigate these effects?
Does deep versus moderate hypothermia affect excitation-contraction coupling and calcium transients in rat ventricular myocytes, and does dantrolene mitigate these effects?
Deep hypothermia disrupts excitation-contraction coupling in rat myocardium, an effect that can be partially mitigated by RyR2 antagonism with dantrolene.
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Hypothesis-generating for dantrolene in hypothermic myocardial protection; clinical translation requires prospective human studies.
Groban et al. (2002) studied this question. Deep hypothermia (10-16°C) and Dantrolene vs. Moderate hypothermia (28°C) and control temperatures (32°C) was evaluated on Papillary muscle contractility and myocyte calcium transients upon rewarming. Deep hypothermia disrupts excitation-contraction coupling upon rewarming, but pretreatment with dantrolene preserved calcium transients to 89% of control values compared to 50% without treatment.
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