Key result
ANGPTL4 increases extracellular VLDL while cutting intracellular triglycerides in bovine hepatocytes.
Why the study?
It remains unclear whether ANGPTL4 is involved in the pathological process of fatty liver in dairy cows through regulation of hepatocellular lipid transport.
Does ANGPTL4 promote VLDL assembly and secretion in bovine hepatocytes?
Does ANGPTL4 promote VLDL assembly and secretion in bovine hepatocytes?
ANGPTL4 promotes the transport of triglycerides in the form of VLDL in bovine hepatocytes, contributing to the adaptive regulation of lipid transport in dairy cows.
May inform bovine lipid metabolism; leaves open translation to human dyslipidemia or therapy.
In dairy cows, fatty liver is one of the most common metabolic diseases that occurs during the periparturient period. Angiopoietin-like protein 4 (ANGPTL4) is a well-known downstream target of peroxisome proliferator-activated receptors (PPARs), which regulate the glucose and fatty acid metabolisms. The inhibition of lipoprotein lipase (LPL) activity interferes with the storage of triglycerides (TG) in adipocytes, which plays an essential role in lipid metabolism in rodents. However, it remains unclear whether ANGPTL4 is involved in the pathological process of fatty liver in dairy cows as a result of the regulation of the hepatocellular lipid transport system. This study intended to investigate the effect of ANGPTL4 on the very-low-density lipoprotein (VLDL) assembly and secretion in bovine hepatocytes. Bovine hepatocytes were isolated using a modified two-step perfusion and collagenase digestion process, and treated with different concentrations of ANGPTL4 (0, 4, 12, and 24 ng/ml) for 24 hr. The results showed that a high concentration of ANGPTL4 could significantly increase the extracellular concentration of VLDL while reducing the intracellular content of TG. Thus, it was confirmed that ANGPTL4 could promote the transport of TG in the form of VLDL by partially regulating the expression of related proteins in hepatocytes, thereby contributing to the partial adaptive regulation of lipid transport in dairy cows.
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Kong et al. (2020) studied Fatty liver in dairy cows (in vitro model). Angiopoietin-like protein 4 (ANGPTL4) vs. 0 ng/ml was evaluated on Extracellular concentration of VLDL and intracellular content of TG. High concentrations of ANGPTL4 significantly increased the extracellular concentration of VLDL while reducing the intracellular content of TG in bovine hepatocytes in vitro.
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