Key result
Host smooth muscle cell accumulation in cardiac allografts is linked to higher rejection grade.
Why the study?
The relationship between clinical factors and the presence of host-derived smooth muscle cells in cardiac allografts and the role of inflammation and MCP-1 in their accumulation was unclear.
Observational (n=26)
Investigators blinded to clinical information during data analysis
No
p-value: p=0.034
Minor host-derived SMC contribution in sex-mismatched transplants; hypothesis-generating for recipient cell role without practice implications.
Transplant arteriosclerosis is characterized by inflammation and intimal thickening caused by accumulation of smooth muscle cells (SMCs) both from donor and recipient. We assessed the relationship between clinical factors and the presence of host-derived SMCs in 124 myocardial biopsies from 26 consecutive patients who received hearts from opposite-sex donors. Clinical and demographic information was obtained from the patients' medical records. Host-derived SMCs accounted for 3.35+/-2.3% of cells in arterioles (range, 0.08-12.51%). As shown by linear regression analysis, an increased number of SMCs was associated with rejection grade (mean, 1.41+/-1.03, p = 0.034) and the number of leukocytes (19.1+/-12.7 per 20 high-power fields, p = 0.01). The accumulation of host-derived SMCs was associated with an increased number of leukocytes in the allografts. In vitro, monocyte chemoattractant protein 1 (MCP-1) released from leukocytes was crucial for SMC migration. After heart allotransplantation, mice treated with MCP-1-specific antibodies had significantly fewer host-derived SMCs in the grafts than mice treated with isotypic antibody controls. We conclude that the number of host-derived SMCs in human cardiac allografts is associated with the rejection grade and that MCP-1 may play pivotal role in recruiting host-derived SMCs into cardiac allografts.
No takes yet. Share an insight, caveat, or question.
Religa et al. (2009) conducted an observational in Transplant arteriosclerosis / Cardiac allograft rejection (n=26). Inflammation (higher rejection grade and leukocyte infiltration) vs. Lower rejection grade or leukocyte infiltration was evaluated on Accumulation of host-derived smooth muscle cells (SMCs) in arterioles (p=0.034). The accumulation of host-derived smooth muscle cells in human cardiac allografts was significantly associated with higher rejection grade (p=0.034) and increased leukocyte infiltration (p=0.010).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: