Key result
Circ_0003748 promotes rheumatic valvular heart disease progression by sponging miR-577 to alter valve cell behavior.
Why the study?
The diagnosis and treatment of rheumatic valvular heart disease require substantial improvements and the role of circRNAs in its progression is under investigation.
Cell findings implicate hsa_circ_0003748 in valvular interstitial cell behavior; leaves open in vivo validation and clinical relevance.
The diagnosis and treatment of rheumatic valvular heart disease (RVHD) require substantial improvements. Studies found that circular RNAs (circRNAs) are involved in the progression of cardiovascular diseases. We screened target hsa_circ_0003748 by circRNA microarrays uploaded to a database. We used fluorescence in situ hybridization to determine the cellular location of hsa_circ_0003748. A dual-luciferase reporter gene assay revealed that has_circ_0003748 might bind the miRNA miR-577. In hVIC cells (an RVHD cell line), Cell Counting Kit-8, Transwell, and flow cytometry assays measured proliferation, migration, and cell cycle and apoptosis, respectively. We found that hsa_circ_0003748 was localized in the cytoplasm; hsa_circ_0003748 promoted the proliferation and migration of hVIC cells, arrested the cell cycle in the G2/M phase, and inhibited apoptosis. These phenomena may result from hsa_circ_0003748 promoting RVHD after sponging miR-577. Bioinformatic analysis revealed that hsa_circ_0003748 might affect RVHD progression by affecting transcription and the MAPK signaling pathway, the Ras signaling pathway, the cAMP signaling pathway, the Rap1 signaling pathway, and other signaling pathways.
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Zhang et al. (2022) studied Rheumatic valvular heart disease (RVHD). hsa_circ_0003748 silencing and overexpression vs. Negative control siRNA or empty vector was evaluated on Cell proliferation, migration, apoptosis, and cell cycle. In hVIC cells, hsa_circ_0003748 promoted cell proliferation and migration, and inhibited apoptosis by sponging miR-577, suggesting it promotes the progression of rheumatic valvular heart disease.
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