Key result
Gelatin-trap ultracentrifugation yields ~50% enterovirus detection at titers down to 0.025 PFU/ml.
Why the study?
Ultracentrifugation was evaluated to improve concentration and detection sensitivity of enteroviruses from suspensions with low viral titers.
Answers are grounded in this paper and cite their sources.
May enable enterovirus detection in dilute samples; leaves open clinical adoption pending validation studies.
Ultracentrifugation has been evaluated as a method of concentrating enteroviruses from suspensions whose initial titers ranged from 1.7 x 10(8) to 1.6 x 10(-2) plaque-forming units (PFU) per ml. A technique employing a "trap" of 0.1 ml of 2% gelatin solution at the point at which the pellet forms in tubes for the number 30 and number 50 rotors of the Spinco model L preparative ultracentrifuge has been tested and found to have a number of advantages. Qualitative studies have been performed to determine the sensitivity of the ultracentrifuge technique in detecting the presence of enteroviruses in very dilute suspensions. There was found to be at least a 50% probability of detecting virus present initially at levels as low as 0.12 PFU per ml by means of the number 50 rotor. The input level for similar results with the number 30 rotor was found to be 0.025 PFU per ml.
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Cliver et al. (1965) studied Enterovirus detection. Ultracentrifugation with a 2% gelatin trap was evaluated on Probability of detecting virus in dilute suspensions. Ultracentrifugation using a 2% gelatin trap demonstrated at least a 50% probability of detecting enteroviruses in suspensions with titers as low as 0.025 PFU per ml using a number 30 rotor.
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