Phytochemical Profiling, Diabetic Enzymes (Α-Glucosidase, Α-Amylase), and Tyrosinase Inhibitory Activities of Azadirachta Indica Methanol Extract

Azadirachta indica, a medicinal plant widely recognized for its diverse phytochemical constituents and broad spectrum of biological activities, was investigated for phytochemical profile and some biological activities in an in vitro system.Phytochemical profiling was performed on thin-layer chromatography on silica gel plates, with visualization under ultraviolet light at 254 and 366 nm, followed by acid spray detecting reagents, while biological activities such as diabetic causative enzymes (α-amylase and α-glucosidase), and skin degenerative enzyme (tyrosinase) were investigated spectrophotometrically at prescreened effective concentration of 100 μg/mL.The chromatographic profile revealed active constituents, with colour reactions suggestive of flavonoids, sterols, polyphenols, and terpenoids. Phytochemical Profiling, Diabetic Enzymes (Α-Glucosidase, Α-Amylase), and TyrosinaseInhibitory Activities of Azadirachta Indica Methanol Extract