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The final phases of the Global Polio Eradication Initiative are challenged by the circulation of vaccine-derived polioviruses, particularly serotype 2, occurring even in certified polio-free countries. Integrated environmental surveillance and long-read deep sequencing enable resolution of the viral haplotype network. Structural modelling of this network reveals the poliovirus phenotypic landscape. Together, these approaches uncover the complete poliovirus quasispecies in a population of approximately 1.8 million inhabitants.
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Carcereny et al. (2026) studied this question.
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