Constructing an in vitro choriocarcinoma model by three-dimensional bioprinting

Background: choriocarcinoma model and to comprehensively compare it with conventional two-dimensional (2D) culture systems in multiple aspects. Methods: choriocarcinoma model, and the cells in this model were comprehensively compared with those in traditional 2D cultured cells. A fluorescence live/dead kit was used to assess cell viability. Proliferation of JEG-3 cells was assessed by the Cell Counting Kit-8 (CCK-8) assay. We also analyzed the gene expression of 2D and 3D bioprinted cells. Finally, we used antitumor drugs to conduct drug response tests on the cells in the 2D and 3DP groups. Results: ) values for three tested chemotherapeutic agents were substantially elevated in the 3D bioprinted model relative to the 2D model. Conclusions: tumor microenvironment more effectively. The 3D bioprinted microtissue model provides a realistic platform for studying cancer cell behavior and holds significant promise for preclinical applications, particularly in advancing our understanding of choriocarcinoma. This approach is adaptable to other tumor types and could significantly contribute to the development of personalized therapeutic strategies.