Proximate Composition, Minerals Contents and Antioxidants Activities of Hippophae rhamnoides (Sea buckthorn Berries) Extracts

The present study was designed to assess the proximate content, minerals analysis and In-Vitro antioxidant potential of Hippophae rhamnoides. Proximate compositions (fiber, energy value, moisture, ash, protein, carbohydrate and far) were determined applying standards official procedures. Minerals determinations were analyzed using wet digestion methods followed by using Flame Photometer and Atomic Absorption Spectrophotometer. The proximate composition results of H. rhamnoides showed the contents as: moisture (80±01%), ash (1.3±0.1%), fat (2.3±0.2%), fiber (6.4±0.5%), protein (2.9±0.3%), carbohydrate (7.1±0%) and energy value (60.7±0 Kcal/100g fresh wt). the minerals composition (mg/100g) showed the results that potassium (580±01), sodium (325±01), calcium (150±01), phosphorus (70.2±0.1), iron (30±0.5), magnesium (15±0.5), zinc (2.20±0.1), manganese (1.90±0.1), selenium (0.2±00) and copper (1.10±0.1). The ea buckthorn berries extracts studied by five models In Vitro antioxidant test systems as 1,1-diphynyl- 2-picrylhydrazyl (DPPH), Superoxide dismutase (SOD)-like activity, Nitric oxide (NO) scavenging activity, H2O2 scavenging activity and Ferric thiocyanate (FTC) method using ascorbic acid as standard. The results showed that methanol extract at 2 mg/mL remarkably inhibited 1,1-diphynyl- 2-picrylhydrazyl inhibition activity (90±2%), superoxide dismutase-like activity (95±2%), H2O2 antiradical activity (90±1%) and scavenging activity of nitric oxide (75±1%). Similarly, methanol extract in FTC activity showed significant antioxidant potential. The results indicate that H. rhamnoides berries extracts act as potential source of nutrition, minerals and potential scavenging properties which have beneficiary outcomes on the health of human beings. Therefore this plant should be utilized for the treatment of diseases caused by ROS.