Figure S2 from Recharacterization of the Tumor Suppressive Mechanism of RSL3 Identifies the Selenoproteome as a Druggable Pathway in Colorectal Cancer

Stephen L. DeAngelo; Liang Zhao; Sofia Dziechciarz; Myungsun Shin; Sumeet Solanki; Andrii Balia; Marwa O. El-Derany; Cristina Castillo; Yao Qin; Nupur K. Das; Hannah N. Bell; João A. Paulo; Yuezhong Zhang; Nicholas J. Rossiter; Elizabeth C. McCulla; Jianping He; Indrani Talukder; Wai‐Lung Ng; Zachary T. Schafer; Nouri Neamati; Joseph D. Mancias; Markos Koutmos; Yatrik M. Shah

doi:10.1158/0008-5472.29761181

Key Points

Core mechanisms of RSL3's tumor suppressive function are recharacterized, revealing new insights.
GPX4 knockdown effectiveness is validated, showing significant changes in antioxidant enzyme activities.
Kinetic assays illustrate thioredoxin reductase activity dynamics in response to RSL3 and auranofin treatments.
Findings indicate a potential new therapeutic avenue through targeting the selenoproteome in colorectal cancer.

Implication

Supplementary figure reveals GPX4 knockdown impact on antioxidant enzyme activity in colorectal cancer cells, highlighting new treatment prospects.

Abstract

Supplementary Figure S2 validates GPX4 knockdown efficacy, details antioxidant enzyme activities under RSL3 and auranofin treatments, and includes kinetic assays for thioredoxin reductase activity in CRC cells.

Discussion

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Cite This Study

DeAngelo et al. (2025) studied this question.

synapsesocial.com/papers/689a0c6be6551bb0af8cfc97 https://doi.org/10.1158/0008-5472.29761181

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Figure S2 from Recharacterization of the Tumor Suppressive Mechanism of RSL3 Identifies the Selenoproteome as a Druggable Pathway in Colorectal Cancer

Key Points

Implication

Abstract

Discussion

Cite This Study

Figure S2 from Recharacterization of the Tumor Suppressive Mechanism of RSL3 Identifies the Selenoproteome as a Druggable Pathway in Colorectal Cancer

Key Points

Implication

Abstract

Discussion

Cite This Study

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