What type of study is this?

This is a Animal Study study (also classified as: Observational).

Menthol as a sustainable alternative anaesthetic for adult zebrafish (Danio rerio)

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Analysis reveals menthol can induce anaesthesia effectively in zebrafish, suggesting a sustainable method with fewer side effects.

Menthol at 50 mg/L effectively induces anaesthesia in zebrafish, supporting animal welfare in research.
Induction and recovery times were under 400 seconds, comparable to traditional anaesthetics such as MS-222.
Immersion concentration below 25 mg/L failed to induce anaesthesia, while above 150 mg/L showed aversive behaviors.
No mortality was observed at effective concentrations, highlighting the safety of menthol as an anaesthetic.

Félix et al. (2025) studied this question.

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