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September 5, 2025Fish Physiology and BiochemistryOpen Access

Evaluation of thymol application for anaesthesia of adult zebrafish

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Authors

LFLuís FélixSMSandra M. MonteiroCVCarlos Venâncio

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Overview

This study examines the anaesthetic effects of thymol on zebrafish, suggesting welfare-friendly options for aquatic research.

Key Points

  • Thymol at 50 mg/L effectively induces deep anaesthesia in adult zebrafish without causing mortality.
  • Mean induction times at 50 mg/L are around 300 seconds, comparable to MS-222 and eugenol.
  • Increased thymol concentrations revealed adverse effects, indicating safety margin concerns in anaesthetic applications.
  • Further exploration is needed to clarify thymol's physiological impacts and mechanisms in zebrafish.

Cite This Study

Félix et al. (2025) studied this question.

synapsesocial.com/papers/68bb49d26d6d5674bccffe92https://doi.org/10.1007/s10695-025-01574-z
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Anaesthetic profile of thymol and menthol in zebrafish larvae model2024 · 4 citations
  2. 2Menthol as a sustainable alternative anaesthetic for adult zebrafish (Danio rerio)2025
  3. 3Evaluating anaesthetics for improving scientific research and welfare using larval zebrafish2025 · 2 citations
  4. 4Evaluating Anesthetics for Improving Scientific Research and Welfare Using Larval Zebrafish2026
  5. 5Evaluation of the Effectiveness of Eugenol and MS-222 as Anesthetics in Zebrafish in Repeated Exposures and Post-Anesthesia Behaviour2024 · 14 citations