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The aim is to establish suitable anesthesia protocols for larval zebrafish to enhance animal welfare and research efficacy.

Evaluating Anesthetics for Improving Scientific Research and Welfare Using Larval Zebrafish

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The aim is to establish suitable anesthesia protocols for larval zebrafish to enhance animal welfare and research efficacy.
Evaluation of six anesthetics using whole-brain functional imaging, cardiovascular performance assessment, and behavioral analysis.
Comparison of anesthetic efficacy and tolerability specifically in larval zebrafish.
MS222 and quinaldine sulfate were found to be the most effective anesthetics for deep anesthesia.
Etomidate is preferable for studies targeting cardiovascular functions.
Quinaldine sulfate was identified as aversive, impacting its usability.

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Randomized trial evaluates anesthetics for effective research outcomes in larval zebrafish, suggesting optimal compounds for studies.

Dimitriadou et al. (2026) studied this question.

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