This protocol demonstrates whole genome sequencing and characterizes foot-and-mouth disease virus using SISPA, indicating its utility for clinical samples.
This protocol describes a SISPA (Sequence-Independent Single Primer Amplification) approach combined with Oxford Nanopore sequencing for the detection and characterization of Foot-and-Mouth Disease Virus (FMDV) from clinical samples or isolates. We hope this will be useful for other teams working on FMDV. Feel free to reuse, adapt, or cite this protocol using the DOI provided.
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Gondard et al. (2025) studied this question.
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