Key result
Next-generation sequencing of foot-and-mouth disease virus from a single infected bovine host revealed extensive within-host viral population diversity, identifying up to 2,622 polymorphisms and estimating a maximum genome-wide mutation rate of 7.8 × 10^-4 per nucleotide.
Population
A single bovine host inoculated intradermolingually with 10^5.7 TCID50 of foot-and-mouth disease virus and…
Design
Preclinical
Follow-up
2 days postinoculation
Authors
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Indicates high intra-host FMDV diversity in cattle; leaves open implications for viral evolution and livestock control strategies.
Next-generation sequencing reveals extensive within-host viral population diversity and intermediate evolutionary stages of foot-and-mouth disease virus.
Wright et al. (2010) studied Foot-and-Mouth Disease Virus (FMDV) infection (n=1). Next-generation genome sequencing (Illumina) vs. Inoculum / Sanger sequencing was evaluated on Number of validated polymorphisms and genome-wide mutation rate. Next-generation sequencing of foot-and-mouth disease virus from a single infected bovine host revealed extensive within-host viral population diversity, identifying up to 2,622 polymorphisms and estimating a maximum genome-wide mutation rate of 7.8 × 10^-4 per nucleotide.
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