September 30, 2014BMC GenomicsOpen Access

A universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated RNA viruses using the Illumina MiSeq

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Structured PICO

Population

Foot-and-mouth disease virus (FMDV) and other positive-sense polyadenylated RNA viruses

Intervention

Universal whole-genome sequencing (WGS) method using Illumina MiSeq

Outcome

Generation of consensus-level genome sequences

A new universal WGS protocol enables consensus-level sequencing of FMDV and other polyadenylated RNA viruses without genome-specific PCR.

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Advances accessible WGS for RNA viruses from low-input samples; leaves open validation for routine surveillance.

Logan et al. (2014) studied this question.

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