September 30, 2014Open Access

A universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated RNA viruses using the Illumina MiSeq

Structured PICO

Population

Foot-and-mouth disease virus (FMDV) and other positive-sense polyadenylated RNA viruses

Intervention

Universal whole-genome sequencing (WGS) method using Illumina MiSeq

Outcome

Generation of consensus-level genome sequences

A new universal WGS protocol enables consensus-level sequencing of FMDV and other polyadenylated RNA viruses without genome-specific PCR.

Abstract

We have developed a universal WGS method for FMDV and other polyadenylated RNA viruses. This method works successfully from a limited quantity of starting material and eliminates the requirement for genome-specific PCR amplification. This protocol has the potential to generate consensus-level sequences within a routine high-throughput diagnostic environment.

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