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September 10, 2025Halalsphere

Detection of Rusa spp. DNA in ready-to-eat food (RTF) using singleplex of Polymerase Chain Reaction

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Authors

NINooratiny IshakKKKumara Thevan Krishnan

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Overview

Singleplex PCR detects deer DNA in RTE food, highlighting issues of food fraud and halal certification.

Key Points

  • Detection of deer DNA in ready-to-eat food products demonstrates the effectiveness of polymerase chain reaction techniques.
  • The specificity trial identified deer DNA in four out of eight tested food samples, confirming the assay's reliability in detecting adulteration.
  • The detection limit of 0.0001 ng/μL indicates high sensitivity of the PCR assay used for identifying Rusa spp. DNA targets.
  • This detection method plays a crucial role in regulatory enforcement and consumer protection in Malaysia's growing RTE market.

Cite This Study

Ishak et al. (2025) studied this question.

synapsesocial.com/papers/68c1a40954b1d3bfb60de802https://doi.org/10.31436/hs.v5i2.118
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