Supplemental Figure 4 from Augmenting CAR T-cell Functions with LIGHT

(A) Violin plots showing quality metrics of single cells included in downstream analysis. nCount RNA: number of RNA unique molecular identifiers (UMI); nFeature RNA: number of detected genes; nCount_ADT: number of antibody UMI; nFeature_ADT: number of detected antibodies; percent.mt: percentage of mitochondrial gene expression; HTO_margin: difference between signals for the hashtag with the highest signal and the hashtag with the second highest signal. (B) (C) Weighted-nearest neighbor (WNN) UMAP of single cells colored by condition (CAR T cell construct and timepoint) (top) and CD4+ vs CD8+ cell type (bottom). Expression of T cell markers (CD3, CD4, and CD8) at the RNA (top) and antibody-derived tag (ADT) level projected on WNN UMAPs. (D) Expression of the RNA (top) and ADT (bottom) markers of T cell type, proliferation, activation, cytotoxicity, and cytokines support CITE-seq cluster annotation. Blue is control CAR T cell and Red is LIGHT-CAR T cell.