Supplemental Figure 5 from Augmenting CAR T-cell Functions with LIGHT

(A) CAR T cells infiltration and expression of LIGHT at the tumor site was quantified by flow cytometric analysis on day 14 post CAR T cell treatment. (B) (C) Quantification of CAR T cells per gram of AsPC1 tumor mass on day 14 post CAR T cell treatment. Plot is representative of 4 to 5 mice per treatment group. Immunohistochemistry staining of mesothelin-negative cancer cell line, Panc1. Negative control. (D) Immunohistochemistry staining of mesothelin-positive cancer cell line, MDA-MB-231. Positive control. (E) Immunohistochemistry staining of various PDX slides samples to validate their mesothelin expression. (F) Flow cytometric analysis of mesothelin expression in PDAC2, one of the PDX selected for in vivo model. (G) In vitro cytotoxicity assay of PDX PDAC2 cocultured with various CAR T cell constructs. Plot represents 2 independent experiments of separate donor T cells.