Figure 6 from BRCA Status Dictates Wnt Responsiveness in Epithelial Ovarian Cancer

Decreased tumor growth kinetics and improved survival in mice bearing ID8Trp53−/−;Brca2−/− cells. ID8 mouse ovarian cancer cells were injected intraperitoneally in C57BL/6 mice (n = 6). A, Abdominal width was used as surrogate for intraperitoneal tumor growth; note the significant decrease in tumor growth in mice bearing ID8Trp53−/−;Brca2−/− cells. Data are presented as mean ± SEM. Two-way ANOVA with mixed-effect analysis was used to calculate statistical significance. B, Kaplan–Meier survival curve showing improved overall survival (defined as the day abdominal width reached 3.4 cm) in mice bearing ID8Trp53−/−;Brca2−/− cells. The log-rank test was used to calculate statistical significance. C, Formalin-fixed, paraffin-embedded sections of intraperitoneal tumors were immunostained for β-catenin. D, Quantification of C as detailed in “Materials and Methods” (n = 3); ordinary one-way ANOVA with multiple comparisons was used to calculate statistical significance. n.s., not significant.