Anti-Oxidative Protection of Vitamin E Against Doxorubicin Induced Ovarian Toxicity in Animal Model

Background: Chemotherapy has diverse pathologic consequences on the female reproductive system. Current study aimed to ascertain whether vitamin-E administration shielded female rats against doxorubicin-induced ovarian damage. Methods: This laboratory experimental study was conducted at the Department of Anatomy, Khyber Medical University, Peshawar. In this study, 24 female rats weighing between 200 and 250 gm were used and divided into three groups, the control group, research group-I, and research group-II. All animals got weighed at the start of the study and again prior to their sacrifice. On the 28th day, animals were sacrificed, and organs were taken out. To observe any change in the ovarian follicles, 5 um thick sections were cut on a rotary microtome and stained with Hematoxylin and Eosin (H&E) and Massan Trichome. The SPSS-25 was used to analyse the data. The difference between and within groups was measured using the independent sample t-test and chi square test. p?0.05 was considered statistically significant. Results: Rats in both the control and experimental groups-II showed a substantial rise in mean weight (p<0.05), however rats in experimental group-I showed a significant drop in mean body weight (p>0.05) after the experiments. The number of primordial and secondary ovarian follicles did not differ significantly across groups (p>0.05), however there was a significant difference in the number of primary ovarian follicles, atretic follicles, and nuclear fragments (p<0.05). Conclusion: In female rats, simultaneous administration of vitamin E has demonstrated protective effects against doxorubicin-induced ovarian damage. Pak J Physiol 2024;20(2):41-5