Supplementary Figure 8 from Role of Gut Microbiome in Neoadjuvant Chemotherapy Response in Urothelial Carcinoma: A Multi-institutional Prospective Cohort Evaluation

Supplementary Figure 8: Microbial Composition Differences in Mice Exposed to BBN Versus Water Water Group: A: Alpha diversity indices (ACE, Chao1, Shannon) plotted over multiple time points (0, 6, 12, 16-22 weeks), showing variations in microbial diversity in mice given water. B: Line graph depicting the percentage composition of bacterial families over time. C: Venn diagram highlighting the unique and shared bacterial operational taxonomic units (OTUs) at different time points. D: Principal Coordinates Analysis (PCoA) based on weighted UniFrac distances. E: Bar charts representing the relative abundance of specific taxa, identified as significantly different by LEfSe analysis, across the time points. The bars indicate changes in key microbial taxa that are statistically significant. BBN Group: F: Alpha diversity indices (ACE, Chao1, Shannon) for the BBN group. G: Line graph showing the dynamic changes in the percentage composition of bacterial families over time in the BBN-treated mice. H: Venn diagram detailing unique and shared bacterial OTUs between the different time points in the BBN group, illustrating the impact of the carcinogen on microbial turnover. I: PCoA plot for the BBN group, using weighted UniFrac distances to show clustering patterns that differ from the water group. J: LEfSe analysis bar charts showing the relative abundance of significantly different taxa across time points in the BBN group.