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April 30, 2024Scientific ReportsOpen Access

A high efficiency precision genome editing method with CRISPR in iPSCs

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Authors

ASAvinash SinghGSG. Dalton SmedleyJRJ. Rose

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Overview

Experimental study demonstrates high-efficiency homologous recombination in human induced pluripotent stem cells, highlighting streamlined generation of isogenic disease models.

Key Points

  • High-efficiency genetic engineering of point mutations is achieved in induced pluripotent stem cells, facilitating rigorous evaluation of specific gene variants.
  • Homologous recombination rates exceed 90% following dual p53 inhibition and pro-survival small-molecule supplementation, yielding robust precision gene insertion.
  • Protocol using CRISPR and small-molecule cocktails accelerates the generation of isogenic lines, although validation remains restricted to in vitro stem cell models.

Cite This Study

Singh et al. (2024) studied this question.

synapsesocial.com/papers/68e6ca92b6db643587648f50https://doi.org/10.1038/s41598-024-60766-4
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