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Experimental study demonstrates high-efficiency homologous recombination in human induced pluripotent stem cells, highlighting streamlined generation of isogenic disease models.
The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous recombination rate higher than 90% using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in human iPSCs. Our protocol reduces the effort and time required to create isogenic lines.
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Singh et al. (2024) studied this question.
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