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November 12, 2025Advanced Functional MaterialsOpen Access

Nanopuncturing‐Enabled Efficient Gene Editing for Isogenic Human iPSC‐Derived Organoid Engineering

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Authors

MGMuhammad Waseem GhaniYKYutong KongAIAmbreen Iqbal

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Overview

In vitro study demonstrates high-efficiency CRISPR delivery via silicon nanoneedles in human induced pluripotent stem cells, highlighting an effective platform for isogenic organoid disease modeling.

Key Points

  • To establish a nanoneedle-assisted delivery platform that enables high-efficiency CRISPR/Cas9 gene editing in human induced pluripotent stem cells for constructing isogenic organoids.
  • Fabricated a silicon nanoneedle array to mechanically pierce human induced pluripotent stem cell membranes and introduce CRISPR/Cas9 plasmids within a 30-minute operational window.
  • Targeted the PTPRB and ZNF521 genes to evaluate knockout efficiency and determine impacts on stem cell pluripotency and organoid differentiation potential.
  • Nanopuncturing produced a plasmid transfection efficiency of 45 ± 4%, representing a nearly fivefold improvement over conventional lipid-based reagents.
  • Demonstrated high plasmid expression across transfected cells and successfully knocked out PTPRB and ZNF521 without impairing stemness or multilineage differentiation capacity.

Cite This Study

Ghani et al. (2025) studied this question.

synapsesocial.com/papers/6aa01e4e0350eeaf1dbe81a7https://doi.org/10.1002/adfm.202523579
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  1. 1Nanopuncturing‐Enabled Efficient Gene Editing for Isogenic Human iPSC‐Derived Organoid Engineering2025
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